MIP-1α– and MIP-1β–mediated OVA-specific CTL responses.

Groups of 5 C57BL/6 mice were nasally immunized on days 0, 7, and 14 with 75 μg OVA and 0.0 (▪) or 1.0 μg MIP-1α (♦) or MIP-1β (●) in PBS. One week after the last immunization, spleen-, Peyer patch–, and CLN-derived CD8⁺ lymphocytes were purified and restimulated ex vivo with OVA peptide 257-264 (SIINFEKL) for 5 days with syngeneic EL4 cells in complete medium. Experimental groups consisted of 5 mice, and studies were repeated 3 times. The cytotoxic response was determined by 4-hour ⁵¹Cr release against E.G7.OVA or OVA peptide 257-264 (SIINFEKL) pulsed EL4 cells. Data shown are mean values of triplicates obtained at varying effector-target ratios as indicated.