MIP-1α and MIP-1β differentially regulate Ag-specific Th cytokine responses.
Groups of 5 C57BL/6 mice were intranasally immunized on days 0, 7, and 14 with 75 μg OVA and 0.0 (■) or 1.0 μg MIP-1α (▨) or MIP-1β (▪) in PBS. One week after the last immunization, spleen- (A), Peyer patch– (B), lower respiratory tract (lung and mediastinal lymph nodes)– (C), and CLN-derived (D) T cells were purified and cultured at a density of 5 × 106/mL with 500 μg/mL OVA for 5 days with T cell–depleted, irradiated splenic feeder cells (1 × 106/mL) in complete medium. Experimental groups consisted of 5 mice, and studies were repeated 3 times. Cytokine production of cultured supernatants was determined by ELISA. Th1- and Th2-type cytokine profiles are presented as the mean cytokine levels (picograms per milliliter) ± SEMs of duplicate cultures from each group. Asterisks indicate statistically significant differences (P < .05) from cytokine levels of mice immunized with OVA alone, while cytokines below detectable levels are designated BD.
