OVA-specific serum IgA, IgM, IgG, and IgE Ab responses following nasal immunization with MIP-1α or MIP-1β.

Groups of 5 C57BL/6 mice were nasally immunized on days 0, 7, and 14 with 75 μg OVA and 0.0 (■) or 1.0 μg MIP-1α or MIP-1β (▪) in PBS. The data presented are the mean Ab titers ± SEMs of 3 separate experiments. ELISA determined the distribution of OVA-specific serum and fecal Ab titers on day 21. Asterisks indicate statistically significant differences (P < .05) from Ab titers of mice immunized with OVA alone.