V72M NE expression.

(A) NE mRNA was measured in wild-type and homozygous V72M NE bone marrow cells using a real-time quantitative RT-PCR assay. The amount of NE mRNA transcripts from wild-type (WT) and homozygous (HZ) mice (n = 5, each) were quantified relative to the amount of GAPDH mRNA. NE/CG-deficient mice (KO, n = 2) were used as a negative control. Data are normalized relative to the amount of NE mRNA transcripts in wild-type mice. (B) Functional NE protein in bone marrow cells from wild-type (n = 7), heterozygous (HET, n = 5), homozygous (n = 5), and NE/CG-deficient mice (n = 2) was quantified using a chromogenic, NE-sensitive substrate. The change in optical density at 405 nm (ΔOD₄₀₅) per minute is shown. Data represent the mean ± SEM. *P < .05 compared with heterozygous V72M NE mice. **P < .05 compared to wild-type, heterozygous, and homozygous mice.