ICSBP domain requirements for DC development.

(A) Diagram of ICSBP mutants. The partner interaction/DNA-binding activities and the ability to rescue DC development are summarized on the right. (B) Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis of radiolabeled in vitro translation products tested in panel D. (C) EMSA analysis. In vitro–translated wild-type and mutant ICSBP were mixed with IRF-2 or PU.1 and analyzed for binding with the ISRE or EICE probe. Specificity of binding was confirmed with 100-fold excess unlabeled probe as a competitor (rightmost lane of each blot). (D) ICSBP^−/− cells transduced with the indicated vectors were stimulated by LPS and expression of indicated mRNAs was detected by semiquantitative RT-PCR.