Phenotype of thymic CD11c⁺ B220⁺cells.

(A) LDF was prepared from thymus. Contour plots show the FSC versus SSC profile of CD11c⁺ B220⁺ F4/80⁻ or CD11c⁺ B220⁻ F4/80⁻ cells. These 2 populations were sorted and stained with hematoxylin and eosin (magnification ×100). (B) Histograms show the expression of the indicated markers by thymic CD11c⁺ B220⁺ cells (gray profile). For DEC-205, MHCII, CD40, CD86, CDα, Thy1, CD4, and GR1 empty profiles correspond to CD11c⁺ B220⁻thymic DCs. The empty profiles for CD11b, CD19, and NK1.1 histograms correspond to thymic macrophages (F4/80⁺), thymic B cells (CD11c⁻ B220⁺ F4/80⁻) and thymic NK cells (NK1.1⁺), respectively. Data are representative of 3 experiments.