Analysis of CD11c⁺ B220⁺ cells in different lymphoid tissues.

(A) LDF was prepared from the indicated tissues. Four-color staining was performed with anti-F4/80–Cy5, anti-CD11c–FITC, anti-B220–CyChrome, and anti- MHCII–PE. Contour plots correspond to CD11c versus B220 expression after gating out F4/80⁺ cells. MHCII expression by CD11c⁺ B220⁺ cells was very low compared with CD11c⁺ B220⁻ or CD11c⁻ B220⁺ cells (data not shown). Numbers indicate percentage of cells in the respective quadrant. The data are representative of 3 independent experiments. (B) Bar diagram showing the relative proportion of DCs and PDCs among CD11c⁺ cells in the indicated lymphoid tissue. The data are representative of 3 independent experiments.