Fig. 4.
Fig. 4. CD150 triggering augments IFN-γ production by naive T cells independently of SAP expression. / Wild-type C57Bl/6 or SAP−/− splenic T cells purified by negative selection were stimulated with plate-bound anti-CD3 (145.2C11, 1 μg/mL) and IL-2 at 50 U/mL for 72 hours in the presence or absence of soluble anti-mCD150 (9D1, 10 μg/mL). Following stimulation, IFN-γ concentrations in the cell culture supernatants were measured by ELISA.

CD150 triggering augments IFN-γ production by naive T cells independently of SAP expression.

Wild-type C57Bl/6 or SAP−/− splenic T cells purified by negative selection were stimulated with plate-bound anti-CD3 (145.2C11, 1 μg/mL) and IL-2 at 50 U/mL for 72 hours in the presence or absence of soluble anti-mCD150 (9D1, 10 μg/mL). Following stimulation, IFN-γ concentrations in the cell culture supernatants were measured by ELISA.

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