Fig. 1.
Fig. 1. Construction of Tyr→Phe–substituted KITVal814 and C-terminal–deleted KITVal814. / Murine KIT contains 22 tyrosine residues in the cytoplasmic domain. Each Tyr residue at codons 544, 546, 552, 567, 569, 577, 608, 645, 671, 674, 702, 719, 728, 745, 772, 821, 844, 853, 868, and 878 of KIT Val814 was changed to Phe using the site-directed mutagenesis technique, and the C terminal region (70 amino acids) containing 2 Tyr residues at codons 898 and 934 was deleted from KITVal814 by the introduction of a stop codon. Locations of juxtamembrane domain (juxta), 2 tyrosine kinase domains (TK1, ATP-binding region; TK2, phosphotransferase region), kinase insert (KI), C terminal region (C), and each Tyr residue in the cytoplasmic domain are indicated. Y represents tyrosine (Tyr); F, phenylalanine (Phe).

Construction of Tyr→Phe–substituted KITVal814 and C-terminal–deleted KITVal814.

Murine KIT contains 22 tyrosine residues in the cytoplasmic domain. Each Tyr residue at codons 544, 546, 552, 567, 569, 577, 608, 645, 671, 674, 702, 719, 728, 745, 772, 821, 844, 853, 868, and 878 of KIT Val814 was changed to Phe using the site-directed mutagenesis technique, and the C terminal region (70 amino acids) containing 2 Tyr residues at codons 898 and 934 was deleted from KITVal814 by the introduction of a stop codon. Locations of juxtamembrane domain (juxta), 2 tyrosine kinase domains (TK1, ATP-binding region; TK2, phosphotransferase region), kinase insert (KI), C terminal region (C), and each Tyr residue in the cytoplasmic domain are indicated. Y represents tyrosine (Tyr); F, phenylalanine (Phe).

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