Fig. 9.
Induction of apoptosis of MV 4-11 (FLT3 ITD) cells by SU5416 and SU5614.
Cells were incubated with SU5416 for 72 hours and SU5614 for 48 hours, and apoptosis was assessed by means of a flow cytometric assay of annexin V binding and propidium iodide exclusion. Values represent the percentage of cells demonstrating early or late stages of apoptosis. SU5416 was less potent, inducing less than 40% apoptosis in the cells treated with 2.5 μM. By comparison, SU5614 displayed more marked effects, with an IC50 of approximately 500 nM. No effects are noted when inhibitors are used at concentrations of less than 250 nM.
