Genomic DNA ITD mutation of the juxtamembrane domain of FLT3 in MV 4-11 cells.

MV 4-11 cells are homozygous for genomic DNA ITD mutation of the juxtamembrane domain of FLT3. (A) WAVE nondenaturing HPLC of MV 4-11 FLT3 DNA demonstrated a lower mobility peak indicative of a longer amplicon, characteristic for an ITD mutation. The amplimers for RS 4;11, TF-1, MO7e, and HEL were all of similar size and were sequenced and found to be wild type. (B) The MV 4-11 amplicon was sequenced, and the mutation is shown schematically here. MV 4-11 has a 30-bp (10 amino acid) tandem repeat (boxed). Wild-type FLT3 cDNA sequence is provided for comparison.