Hematopoietic cells expressed MAPA.

(A) Predicted amino acid sequence of MAPA. The predicted signal peptide and the transmembrane domain are boxed in dotted lines. The putative PI3 kinase interaction motif (solid black outline) and the ITAM motif (YXXI/L (7/8) YXXI/L) (dashed underlining) are boxed. Conserved cysteines are circled in red. Potential N-linked glycosylation sites (N) are boxed. Conserved aa are highlighted in yellow. (B) Genomic structure of MAPA gene. Schematic diagram illustrating the relative positions of introns and exons. (C) RT-PCR (35 cycles) was performed using MAPA-specific oligonucleotides (amplifying a product of 515 bp). Results are representative of 1 of 4 experiments. (D) Northern blots of human tissues were analyzed with a probe corresponding to the MAPA PCR product labeled with [³²P]dCTP.