High levels of granzyme B mRNA in PDC and activated T cells.

(A) RT-PCR (35 cycles) was performed using granzyme B–specific oligonucleotides (amplifying a product of 676 bp). Granzyme B is weakly expressed on monocytes, activated MDDCs, B cells, and granulocytes. Results are representative of 1 of 4 experiments. (B) FACS analysis expression of intracytoplasmic granzyme B protein (solid lines) versus isotype-matched control (dashed line). Mean fluorescence intensity of granzyme B staining minus that obtained with the isotype matched control is indicated. Granzyme B protein was clearly detected on PDCs (activated or not) and was weakly expressed in blood monocytes and MDDCs.