Fig. 3.
Fig. 3. Cells of the immune system express Eph-B1. / (A) RT-PCR (35 cycles) was performed using Eph-B1–specific oligonucleotides (amplifying a product of 580 bp) on the same populations as those detailed in Figure 2. Eph-B1 is highly expressed on PDCs and present in B cells and CD34-derived DCs. Results are representative of 1 of 4 experiments. (B) FACS analysis expression of intracytoplasmic Eph-B1 protein on freshly isolated PDCs and MDDCs. (C) Eph-B1 stainings in PDCs (freshly isolated and cultured overnight in IL-3) and MDDCs on cytospin with (iii,iv) or without (i,ii,v,vi) hematoxylin counterstaining. Specific inhibition in the presence of Eph-B1 peptide (ii,iv) of the labeling using a rabbit polyclonal antibody generated against an intracytoplasmic peptide of Eph-B1 (i,iii). Original magnifications: (i,ii,v,vi) × 400; (iii,iv) × 1000.

Cells of the immune system express Eph-B1.

(A) RT-PCR (35 cycles) was performed using Eph-B1–specific oligonucleotides (amplifying a product of 580 bp) on the same populations as those detailed in Figure 2. Eph-B1 is highly expressed on PDCs and present in B cells and CD34-derived DCs. Results are representative of 1 of 4 experiments. (B) FACS analysis expression of intracytoplasmic Eph-B1 protein on freshly isolated PDCs and MDDCs. (C) Eph-B1 stainings in PDCs (freshly isolated and cultured overnight in IL-3) and MDDCs on cytospin with (iii,iv) or without (i,ii,v,vi) hematoxylin counterstaining. Specific inhibition in the presence of Eph-B1 peptide (ii,iv) of the labeling using a rabbit polyclonal antibody generated against an intracytoplasmic peptide of Eph-B1 (i,iii). Original magnifications: (i,ii,v,vi) × 400; (iii,iv) × 1000.

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