Restricted expression profile of ILT7.

RT-PCR (35 cycles) was performed using ILT7-specific oligonucleotides (amplifying a product of 973 bp) on first-strand cDNA from freshly isolated PDCs or activated (act) at different time points (from donors different from the one used for the subtraction; see “Materials and methods”); monocytes; MDDCs nonactivated (day 6) or activated (in the presence of CD40L-transfected cell line and GM-CSF) at different time points; on resting or activated T cells, B cells, and granulocytes; and on CD34-derived DCs. ILT7 was expressed mainly in PDCs, monocytes, and activated B cells. Results are representative of 1 of 2 experiments.