Fig. 1.
Fig. 1. Differential mRNA expression of selected genes between PDCs and MDDCs. / CD62L, CD123/IL-3Rα, CXCR4, λ light chain, surrogate κ light chain, J chain, Spi-B, ILT7, Eph-B1, and granzyme B expression patterns were compared with reverse-transcribed RNA from PDC (a mix of 50% freshly isolated PDCs and 50% of IL-3/CD40L-activated PDCs) and MDDCs (CD40-activated MDDCs) that was kept from the original material used for the subtraction. RT-PCR consisted of 35 cycles of denaturing (30 seconds, 94°C), annealing (30 seconds), and extension (2 minutes, 72°C). Lack of B-cell contamination in PDCs and MDDCs was verified using CD19 and VHconsensus-Cμ primers previously validated on cDNA from tonsil B cells. GAPDH-specific oligonucleotides were used on the same populations.

Differential mRNA expression of selected genes between PDCs and MDDCs.

CD62L, CD123/IL-3Rα, CXCR4, λ light chain, surrogate κ light chain, J chain, Spi-B, ILT7, Eph-B1, and granzyme B expression patterns were compared with reverse-transcribed RNA from PDC (a mix of 50% freshly isolated PDCs and 50% of IL-3/CD40L-activated PDCs) and MDDCs (CD40-activated MDDCs) that was kept from the original material used for the subtraction. RT-PCR consisted of 35 cycles of denaturing (30 seconds, 94°C), annealing (30 seconds), and extension (2 minutes, 72°C). Lack of B-cell contamination in PDCs and MDDCs was verified using CD19 and VHconsensus-Cμ primers previously validated on cDNA from tonsil B cells. GAPDH-specific oligonucleotides were used on the same populations.

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