![Fig. 7. Phosphorylation of overexpressed hTRPC1 and mTRPC6 using CAT of cAMP-PK. / 500 μg membrane proteins from QBI-293A cells overexpressing either hTRPC1 or mTRPC6 were incubated with γ[32P] ATP in the absence of CAT (CON), presence of 350 U CAT (+PKA CAT), or presence of 1 μM cAMP-PK inhibitor peptide (+PKA INHIB). (A) Autoradiography of Ank immunoprecipitations of overexpressed hTRPC1 and subsequent probing of the Western blot with the Ank antibody (5 μg/mL). (B) Autoradiography of anti-TRPC6 immunoprecipitations of overexpressed mTRPC6 and probing with anti-TRPC6 antibody (1/400). Note both partial and fully glycosylated forms of mTRPC6 undergo phosphorylation. Molecular size markers are noted in kilodaltons (kDa) and the TRPC protein identifications indicated with arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/8/10.1182_blood-2002-03-0723/4/h82023238007.jpeg?Expires=1724260286&Signature=n1kfV1ZEDgP7pTJqzVMptD2N4vY6ZTeoHMcaIu67VGoiprRh1t00j7VNN7t8DOTcqRE525o2l2KqHmGkZdOsBnm0LcQDvC3oBEsj0cs3pUSrpXx0NwliSbPhXMVGaRuOuqmhzaCojDg7ns2PKacsL922f-lMVw5Vo2PTDvAkZty1Y1XMs9GYCR~6OVE7GmTstRRbjTAXrhpCzcsX7aOZzUBh3OjTetVGoY2jvKni11HtQuFPGJ1OfG0epJnAz6e4UNmVmXAK0Epx9IUMe4~ZekQOXiGKh3Mz~tub~pw7MplwYzwaqobcy5f64tIqjyqDTKesdLQtoR7icISJGpfJ-A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Phosphorylation of overexpressed hTRPC1 and mTRPC6 using CAT of cAMP-PK.
500 μg membrane proteins from QBI-293A cells overexpressing either hTRPC1 or mTRPC6 were incubated with γ[32P] ATP in the absence of CAT (CON), presence of 350 U CAT (+PKA CAT), or presence of 1 μM cAMP-PK inhibitor peptide (+PKA INHIB). (A) Autoradiography of Ank immunoprecipitations of overexpressed hTRPC1 and subsequent probing of the Western blot with the Ank antibody (5 μg/mL). (B) Autoradiography of anti-TRPC6 immunoprecipitations of overexpressed mTRPC6 and probing with anti-TRPC6 antibody (1/400). Note both partial and fully glycosylated forms of mTRPC6 undergo phosphorylation. Molecular size markers are noted in kilodaltons (kDa) and the TRPC protein identifications indicated with arrows.
