Fig. 6.
![Fig. 6. Serine or threonine phosphorylation of TRPC1 and TRPC6. / [32P]Pi-labeled platelets were used in a resting state (R) or incubated with 2 U/mL thrombin (T), 500 μM BIMPS (B), or 500 μM 8PCPT (8P). (A) Autoradiography (of dried-down gel) using 50 μL platelet lysates per lane. (B) Autoradiography of Ank immunoprecipitation blot. (C) Autoradiography of anti-TRPC6 immunoprecipitation blot. In (B) and (C), lower panels represent immune detection of blots with Ank and anti-TRPC6, respectively. Molecular size markers are noted in kilodaltons (kDa), TRPC and coimmunoprecipitated phosphoproteins are indicated with arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/8/10.1182_blood-2002-03-0723/4/h82023238006.jpeg?Expires=1726861416&Signature=O~QgrQuERuh8-vpfkO6pgltrziBJ8Ndq0ugmnO3wPsfEWu9a64bNPUdW7caHapgUfT5QtTGxWVmZMqt17v~pAfzZin0Sk-Ps3bRW3q40nrDJIlTHvZPrAtwe4XMSy9GSK3FDno-pbpiB~hkQZNUqKIKMm5S9TftAq2TQLkfdXPqii9R5ReqQLcje2PtZ~NBqhQYzYUN~zZyth~HEBsfTpX4LzQF3WCo3qUpDCKeW~KE1fyodyVF~HZ8A8vQjipPCJR5VKren0kj-mmuGKyA34zd~-zMmDEnyfOPTFBG3Yl-tvki6tq8mXQ7aRkn58HsQ6fSol0nGigUWPlDch0GL0Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Serine or threonine phosphorylation of TRPC1 and TRPC6.
[32P]Pi-labeled platelets were used in a resting state (R) or incubated with 2 U/mL thrombin (T), 500 μM BIMPS (B), or 500 μM 8PCPT (8P). (A) Autoradiography (of dried-down gel) using 50 μL platelet lysates per lane. (B) Autoradiography of Ank immunoprecipitation blot. (C) Autoradiography of anti-TRPC6 immunoprecipitation blot. In (B) and (C), lower panels represent immune detection of blots with Ank and anti-TRPC6, respectively. Molecular size markers are noted in kilodaltons (kDa), TRPC and coimmunoprecipitated phosphoproteins are indicated with arrows.
