Tyrosine phosphorylation of TRPC1 and TRPC6.

Western blot analysis of lysates from resting platelets (R), platelets activated with 2 U/mL thrombin (T), or activated with 30 μg/mL collagen (C). (A) Western blot analysis of 50 μL platelet lysates and Ank immunoprecipitations probed with the antiphosphotyrosine antibody 4G10 (1 μg/mL) and Ank (5 μg/mL). (B) Western blot analysis of 50 μL platelet lysates and anti-TRPC6 immunoprecipitations probed with the antiphosphotyrosine antibody 4G10 (1 μg/mL) and anti-TRPC6 (1/400). Np reflects control immunoprecipitations with anti-TRPC6 without platelet lysates. Molecular size markers are noted in kilodaltons (kDa) and the TRPC protein identifications indicated with arrows.