Adhesion of clonogenic progenitor cells CFU-GM and BFU-E to laminins and fibronectin.
Bone marrow CD34+ cells were plated in 96-well plates coated with PBS, fibronectin (FN), laminin-1 (LN-1), laminin-8 (LN-8), or laminin-10/11 (LN-10/11). After incubation for 1 hour at a 37°C, 5% CO2, humidified environment, the nonadherent and adherent cells were collected separately and plated in methylcellulose progenitor assay. The percentages of adherent progenitors were calculated as follows: (no. of adherent CFCs/no. of adherent CFCs + no. of nonadherent CFCs) × 100. The total numbers of CFCs adherent to wells coated with PBS were 3672 ± 730, fibronectin 8664 ± 990, laminin-1 4690 ± 340, laminin-8 8408 ± 522, and laminin-10/11 22 553 ± 1635; the values are mean ± range of 2 measurements for laminin-8 and mean ± SD of 3 measurements for other proteins and PBS. Asterisks indicate a significant difference from the control value (percentage of adherent colonies in wells coated with PBS). *P < .05; **P < .01; ***P < .001.
