Fig. 3.
Tyrosine phosphorylation of SHIP upon FcγR stimulation in BMMs from gene-targeted mice.
(A) BMMs (4 × 106) from gene-targeted mice indicated were stimulated with 40 μg/mL ΔIgG for indicated minutes, lysed, and immunoprecipitated with anti–SHIP antibody. The immunoprecipitates were blotted with antiphosphotyrosine antibody (upper panel; anti-pTyr) or anti–SHIP antibody (lower panel). (B) The amount of tyrosine-phosphorylated SHIP to total SHIP shown in panel A was quantified and expressed as fold increase of the ratio. The results were shown as relative values of the time-zero controls and as averages from 2 independent experiments. Bars represent standard errors of duplicate measurements.
