The expression of proteins involved in regulation of the cell cycle is altered in 32D/Jak3 cells stimulated by G-CSF.

(A) 32Dcl3 and (B) 32D/Jak3 cells were washed free of IL-3 and plated in IMDM in the presence of G-CSF. Cells were harvested at the indicated time (days) after exposure to G-CSF and lysed in lysis buffer containing 0.5% Triton X-100. Then, 50 μg of the total cell lysate was used for immunoblotting with p27^Kip1, Cyclin E, Cdk6, Cdk2, and Cdk4 antibodies. Immunoblots were visualized by ECL (Amersham) followed by autoradiography.