Akt and Stat3 pathways are inactivated in 32D/Jak3 cells stimulated by G-CSF.

32Dcl3 and 32D/Jak3 cells were washed free of IL-3 and plated in IMDM in the presence of G-CSF. Cells were harvested at the indicated time (days) after exposure to G-CSF and lysed in lysis buffer containing 0.5% Triton X-100. Then, 50 μg of the total cell lysates was used for immunoblotting with (A) phospho-Stat3 and Stat3, (B) phospho-Stat5, (C) c-myb, and (D) phospho-Akt and Akt.