Northern blot analysis of selected mRNAs.

Equivalent amounts of RNA from MPRO cells induced by ATRA at different time points (0 hour, 24 hours, 48 hours, and 72 hours) were resolved by formaldehyde-agarose gel electrophoresis, stained to verify the amount of loading. Twenty transcription factor genes were separately probed on the RNA filters. The gene symbol of each probe was listed at the left of related Northern blot result. One of the RNA-blotted membrane photographs is shown with methylene blue-stained 28S and 18S RNA subunits demonstrating the quality and quantity of RNA loaded in individual lanes.