Fig. 7.
Fig. 7. Two-dimensional electrophoretograms of cycloheximide inhibition of MPRO cells. / MPRO cells from cycloheximide inhibition experiment were also analyzed by basic pH range 2 DE. MPRO cell lysate (1.5 × 106 cells/sample) was loaded for IPGs-PAGE pH 6 to 11 and stained with brilliant blue G-colloidal dye. (A) Control MPRO cells. (B) Cycloheximide-treated MPRO cells. (C,D) The magnified regions of 2 DE gels shown as inset in panels A and B. The arrowheads point to protein spots that decrease in intensity after cycloheximide treatment; the arrows point to spots whose intensity increases after cycloheximide treatment. The other information is presented as in the legend to Figure 1.

Two-dimensional electrophoretograms of cycloheximide inhibition of MPRO cells.

MPRO cells from cycloheximide inhibition experiment were also analyzed by basic pH range 2 DE. MPRO cell lysate (1.5 × 106 cells/sample) was loaded for IPGs-PAGE pH 6 to 11 and stained with brilliant blue G-colloidal dye. (A) Control MPRO cells. (B) Cycloheximide-treated MPRO cells. (C,D) The magnified regions of 2 DE gels shown as inset in panels A and B. The arrowheads point to protein spots that decrease in intensity after cycloheximide treatment; the arrows point to spots whose intensity increases after cycloheximide treatment. The other information is presented as in the legend to Figure 1.

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