Fig. 5.
![Fig. 5. Functional analysis of ΔCD79b. / Constructs encoding the wild-type ΔCD79b, a leaderless ΔCD79b, a ΔCD79b construct in which the endogenous leader was replaced with that from rat CD4 (CD4L), and the CD4LΔCD79b possessing a mutated ITAM (CD4LΔCD79b [Y207A]) were transfected into Ramos-EHRB cells and then positive clones assessed for sensitivity to anti-μ–induced apoptosis after 48 hours by annexin V–FITC/PI analysis. Two clones from each construct are shown. The average of 3 experiments, ± SD, are shown for each clone. Underneath, expression levels of CD79a, CD79b, and sIg are shown for each clone, determined as indicated in Figure 2, illustrating that all clones expressed similar levels of all BCR components at the cell surface.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/9/10.1182_blood.v100.9.3068/4/h82123348005.jpeg?Expires=1724262253&Signature=IU4L9CdFbDAV1URHFCNaxEt-jGRy3oFSPBLdLKSOMPt1T7CCIoF4voTqx-mR4ptMSmHtyU7xZr8F6LwMQnv5G-DgMhFN5wvGDjSy1xBt~B8UNzezKMM2UhTGq5s4K~aYjgI7TLAKkZMTDrlWJJh3lOun3nPyJMTC-czdPDtCQECfcL5z9Y0AMarUxlAgkqOIX9Ub4uCt1FS9uxKf1EOepDES-0-0MQKU4wtuTGLV5w91QuYhtFG0GE5VPELLs5N38w9tlaUsy9ETsk09b2ea8Kzf6r1VBFEm5KfwyjUiuk1WTdfC2TdEFN7nCb23OoIRKC0OrlSqnSXIMJ2dz1~tkA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Functional analysis of ΔCD79b.
Constructs encoding the wild-type ΔCD79b, a leaderless ΔCD79b, a ΔCD79b construct in which the endogenous leader was replaced with that from rat CD4 (CD4L), and the CD4LΔCD79b possessing a mutated ITAM (CD4LΔCD79b [Y207A]) were transfected into Ramos-EHRB cells and then positive clones assessed for sensitivity to anti-μ–induced apoptosis after 48 hours by annexin V–FITC/PI analysis. Two clones from each construct are shown. The average of 3 experiments, ± SD, are shown for each clone. Underneath, expression levels of CD79a, CD79b, and sIg are shown for each clone, determined as indicated in Figure 2, illustrating that all clones expressed similar levels of all BCR components at the cell surface.
