Transient transfections of HeLa and PC3 cells with luciferase reporter constructs driven by the −30 or −86 region of the human uPA promoter.

The sequences between −86 and +32 (−86 construct) and between −30 and +32 (−30 construct) driving transcription of the luciferase reporter gene are shown in Figure 1. (A) Transient transfections in HeLa and PC3 cells, showing the broad effect of the −86 construct in PC3 cells. (B) Transfection of HeLa and PC3 cells with constructs carrying the uPA enhancer (E −30 and E −86) cloned upstream of the −30 or −86 region. The contribution of the enhancer to transcriptional activation of the reporter construct in PC3 cells is marginal as compared to the effect of the proximal promoter alone (compare A and B).