Fig. 4.
![Fig. 4. Detection of Sp1 and Sp3 in nuclear extracts from HeLa and PC3 cells by overlay with specific polyclonal antibodies of a Western blot analysis. / Proteins from HeLa and PC3 nuclear extracts were fractionated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) (10% [wt/vol] for 2 hours at 150 V) and transferred to a PVDF membrane. The Western blot analysis was sequentially overlaid with specific polyclonal antibodies against Sp1, Sp3, and HMGB-1, and protein detection was performed by chemoluminescence. Sp1 as well as all 3 isoforms of Sp3 are present in HeLa and PC3 cell nuclear extracts in comparable amounts (Table 1).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/9/10.1182_blood.v100.9.3325/4/h82123326004.jpeg?Expires=1724233491&Signature=YExMvHGcynCtC6~wjAGjenKg6K9PSKJC6JeeVBUcs76VqTAoqnTxKyK~GVFDc6whbM4sBjSAMNRcvIAKQPh~yB-KxD7cbwjl62MXf545YBbFUcLE8bxEx8VQU-vbsdDp1gYluJ3psEnxx2tYVkzePDK3TqJcIL2TODKVVrnYM5ss5o2WkhcEuIWgU56KhWx9sPhhpFJTfN-GYvNQq-J~1q1G-rDrJ5WFJos18GaUgWswwBPpR3w1YkeyU-aMaE614Jo-BA07~R8dELaAHVBloNvkuA21QaXFLze9xb6h-vu6tECXNAWof2ifbuXz002PfHmHe5qpBG96jT5MvAMChA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of Sp1 and Sp3 in nuclear extracts from HeLa and PC3 cells by overlay with specific polyclonal antibodies of a Western blot analysis.
Proteins from HeLa and PC3 nuclear extracts were fractionated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) (10% [wt/vol] for 2 hours at 150 V) and transferred to a PVDF membrane. The Western blot analysis was sequentially overlaid with specific polyclonal antibodies against Sp1, Sp3, and HMGB-1, and protein detection was performed by chemoluminescence. Sp1 as well as all 3 isoforms of Sp3 are present in HeLa and PC3 cell nuclear extracts in comparable amounts (Table 1).
