Effect of kallistatin on VEGF- or bFGF-induced angiogenesis in Matrigel implants.

(A) Histologic analysis of implanted Matrigel plugs. Matrigel mixed without or with VEGF or bFGF (150 ng/mL) in the presence or absence of 2 μM kallistatin (HKBP) was injected subcutaneously into mice. The Matrigel plugs were stained with Masson trichrome at 1 week after injection. (B,D) Quantitation of mean capillary area in implanted Matrigel plugs. Each value represents mean area ± SEM (n = 6). (C,E) Hemoglobin levels in implanted Matrigel. Each value represents mean ± SEM (n = 5).