Characterization of CD4⁺CD25⁺ T cells generated from the primed human naive CD4⁺ T cells with allogeneic regulatory DCs.

(A-B) Human allogeneic naive CD4⁺ T cells (5 × 10⁶) were cultured with allogeneic normal or regulatory DCs (5 × 10⁵) for 5 days, and CD4⁺ T cells isolated from the coculture were assayed for phenotype (A) and cytokine profile (B) by FACS. The results are representative of 5 experiments with similar results, and data are represented by a dot plot. (C) Human Ag-primed CD4⁺ T cells were isolated from the coculture of human naive CD4⁺T cells (5 × 10⁶) with allogeneic normal mDCs (5 × 10⁵) for 3 days. CD4⁺CD25⁺T cells were isolated from the coculture of human naive CD4⁺ T cells (5 × 10⁶) with allogeneic regulatory iDCs (5 × 10⁵) for 5 days. Human Ag-primed CD4⁺ T cells or CD4⁺CD25⁺ T cells (10⁵) were cultured with allogeneic normal mDCs (10⁴) in the presence or absence of different numbers of CD4⁺CD25⁺ T cells or Ag-primed CD4⁺T cells, and the proliferative response was measured on day 5. Data were expressed as mean values ± SD of 5 individual experiments. *P < .01 compared with Ag-primed CD4⁺ T cells plus allogeneic normal mDCs by Student paired ttest. Error bars express SD of mean values.