Phenotypic profile of human modified DCs.

(A-B) Cells were stained with the stated mAbs against markers for the DC family molecules (A) and MHC and costimulatory molecules (B) or isotype-matched mAbs, and cell surface expression was analyzed by FACS. Data are represented by a histogram in which cells were stained with the stated mAbs (thick lines) or isotype-matched mAbs (thin lines). The values shown in the flow cytometry profiles are MFI. The results are representative of 10 experiments with similar results. (C) The percent positive cells for MHC and costimulatory molecules were expressed as mean values ± SD of 10 individual experiments. *P < .01 compared with the respective iDCs by Student paired t test. (D) The expression levels of MHC and costimulatory molecules were expressed as MFI ± SD of 10 individual experiments. *P < .01 compared with the respective iDCs or among groups by Student paired ttest.