TCR-γ analysis of both concurrent and sequential MF skin biopsy specimens.

(A) A sample obtained from left-thigh skin showed 2 dominant TCR-γ GRs of 231 and 181 bp that used the VγI and VγIV family primers. (B) A concurrent skin sample obtained from the right thigh showed an oligoclonal pattern with 4 dominant TCR-γ GRs that used VγII and VγIII family primers, respectively. (C) The third specimen, obtained 4 years later from the left thigh (same area as sample shown in A), displayed the same TCR-γ GR of 231 bp that used the VγI family primer. Sequencing confirmed that both GRs using VγI detected 4 years apart were the same. Asterisks indicate TCR-γ GRs. The red peaks without asterisks correspond to internal size standards. Red indicates VγI; blue, VγII; black, VγIII; and green, VγIV.