Fig. 5.
![Fig. 5. Distinct inhibition of [Ca++]ielevations in platelets interacting with immobilized VWF under flow. / A blood cell suspension prepared as described in the legend for Figure2 was perfused for 90 seconds over immobilized VWF at a shear rate of 3000 seconds−1. The diagrams show the [Ca++]i changes in all surface-interacting platelets during translocation or stationary adhesion for 30 seconds. Noteworthy are the presence of α/β peaks and γ peaks (arrows) in the control experiment, the selective absence of γ peaks in the presence of wortmannin (100 nM) or apyrase (10 U/mL), and the absence of any Ca++ elevation in the presence of sodium nitroprusside (5 μM). Similar results were obtained in 4 different experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/8/10.1182_blood-2002-02-0514/4/h82023252005.jpeg?Expires=1724269354&Signature=QNmwo6a3H3JCy9B3B4rZiGUdjFGkb~1msjsP7muEVK~OAp7UzXsNRRUxhdb-JPYzdITYtTfRXsoOXe9XAWlYHkjrnOwqNvqmQSxju-TB1BDw5HtfuVUruCRrn1qc8jTReprYJ4NiWhOiRI2mtVp3OBB0uNVnfQNhoQp32a5GXrGZ7K8EJELhQRkKc0XaK9isUQgpooLr5k2YevrTMLMaeou2ehVs3cCFUM6CCTD~iPymeH~~YSOaVZDToL8CMr0ynImdB-bHhj18OWS9Wa41F368cN-4AuZVj96O0FIJ~Zf6lP~Cq8NXAFIxi9oKUC0nqoSmXGQc16G0kElxYm1KKw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Distinct inhibition of [Ca++]ielevations in platelets interacting with immobilized VWF under flow.
A blood cell suspension prepared as described in the legend for Figure2 was perfused for 90 seconds over immobilized VWF at a shear rate of 3000 seconds−1. The diagrams show the [Ca++]i changes in all surface-interacting platelets during translocation or stationary adhesion for 30 seconds. Noteworthy are the presence of α/β peaks and γ peaks (arrows) in the control experiment, the selective absence of γ peaks in the presence of wortmannin (100 nM) or apyrase (10 U/mL), and the absence of any Ca++ elevation in the presence of sodium nitroprusside (5 μM). Similar results were obtained in 4 different experiments.
