Extensive self-renewal of hemangioblasts in serial single-cell ELTC.

(A) At the left, LTC-IC frequency in primary, secondary, and tertiary ELTCs, as evaluated by limiting dilution (Figure 2H); at the right, serial single-cell ELTC protocol. (B) Microscopy analysis of a very large Hem-End colony generated by blasts grown in tertiary unicellular ELTC (original magnifications: left, × 40; right, × 200). (C) RT-PCR analysis of a Hem-End colony split in 2 halves, which were seeded in secondary endothelial or hematopoietic (not shown) culture.