p210⁺ primary NK cells acquire KIR.

CD56^+bright/CD3⁻ NK cells were prestimulated with IL-2 alone and transduced with p210-eGFP⁺ and eGFP⁺ (control). CD56⁺/eGFP⁺ NK cells were sorted and expanded for 3 weeks, then NK cells were analyzed for expression of at least one KIR, using a cocktail of KIR antibodies against CD158a, CD158b/j, and CD158e. The fold increase in KIR-expressing NK cells was similar from another donor (data not shown).