Serial clones of p210⁺ NK92 cells acquire KIR.

Serial phenotype analysis of p210-eGFP⁺(p210⁺) NK92 clones for KIR expression detected small subpopulations (top row) that expressed CD158b/j allowing selection of subclones. Subclone 1 (CD158b/j⁺; top row, middle panel) continued to acquire KIR (middle row), and subclone 2 expressing CD158e was established (bottom row). The sorting gates shown are representative of those used to establish subsequent subclones.