Fig. 8.
![Fig. 8. Effect of LY294002 on proliferation of human erythroid progenitors and UT7 cells. / Inhibition of PI 3-K/Akt pathway by LY294002 dramatically inhibits proliferation of both human erythroid progenitors and UT7 cells. D7 + 1 human erythroid progenitors and UT7 cells were cultured at 105/mL in serum-free medium with Epo at 2 U/mL, without or with LY294002 (at 50 μM) or rapamycin (at 50 ng/mL) for 48 hours. DNA synthesis was assessed by [3H] thymidine incorporation after 4 hours. Analysis is given as the mean of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/9/10.1182_blood-2002-07-2332/4/h80934214008.jpeg?Expires=1724942313&Signature=31tflAgMP~lEsl8DL957xYYm8j4mIOIYJyluXi-eWvDovlCwKFpPZgU0r5ODlybp5gkB3eLwJOXtc4rYRKVX8wgwolc-AbAwG7gQ0hzEhWkCuH-6b7dM4Xwb~8i1QNqaOkH8gfEG3RxdhsmQ1zaSBX0dNDbISXkCQMxgDLyYm4vq2wElV2UUaCXRRaomLKGecXEmIK5yocNuthzkMeQy1LQF9Si9MMLKvfKC~IR5DJskXkrDGp8DP-91dv5kTaBIee19vX4mGcRhSy56R1feP4LrBX6FdXXONbELciM0ettiERPYY2ODJjtY2USBscCmOk~FU3veoLu0SLMHurdjqA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of LY294002 on proliferation of human erythroid progenitors and UT7 cells.
Inhibition of PI 3-K/Akt pathway by LY294002 dramatically inhibits proliferation of both human erythroid progenitors and UT7 cells. D7 + 1 human erythroid progenitors and UT7 cells were cultured at 105/mL in serum-free medium with Epo at 2 U/mL, without or with LY294002 (at 50 μM) or rapamycin (at 50 ng/mL) for 48 hours. DNA synthesis was assessed by [3H] thymidine incorporation after 4 hours. Analysis is given as the mean of 3 separate experiments.
