LY294002 induces apoptosis in human erythroid progenitors but not in UT7 cells.

Inhibition of PI 3-K by LY294002 induces apoptosis in human erythroid progenitors but not in UT7 cells. D7 + 1 human erythroid progenitors and UT7 cells were plated at 10⁵/mL with 2 U/mL Epo in the absence or presence of LY294002 at 50 μM. Four separate experiments were performed, giving similar results. Results are given for one experiment at 48 hours of incubation with the inhibitor. Controls were obtained from cells cultured in the absence of Epo. Apoptosis was quantified by flow cytometry analysis of the percentage of annexin-V⁺–PE⁺ cells from the whole population.