Fig. 5.
An EpoR without tyrosine residues activates the PI 3-kinase/Akt pathway through IRS2.
Lysates from Ba/F3 or 32D cells expressing either a normal EpoR or a mutant EpoR Zero, stimulated with Epo at 10 U/mL for 10 minutes, in the presence or absence of LY294002 at 50 μM, were analyzed by Western blot with anti-pAkt (Ser473) and anti-Akt antibodies.
