Fig. 1.
Phosphorylated proteins associated with PI 3-kinase in human erythroid progenitors and in UT7 cells stimulated with Epo.
(A) Anti-p85 immunoprecipitates were prepared from 20 × 106 human erythroid progenitors or 15 × 106 UT7 cells stimulated for 10 minutes with 10 U/mL Epo, and analyzed by Western blot with the use of antiphosphotyrosine antibodies (anti-PY). The blot was stripped and reprobed with anti-p85 antibodies. (B) The blot was reprobed with anti-IRS2, anti-SHIP, anti-Gab1 and anti-EpoR, successively.
