A1 expression correlates inversely to Blimp-1 expression in B cells and plasma cells from MM patients and healthy donors.

Semiquantitative gene-specific RT-PCRs were performed to determine relative levels of expression of A1 in B cells and plasma cells from both malignant and healthy subjects. P indicates MM patient; H, healthy donor. (A) (Lane M) A 100 bp ladder (Boehringer Mannheim). (Lane 1) A no-template control. (Lanes 2-9) A1 products (upper lanes) and β-actin products (lower lanes) generated using mRNA templates extracted from percursor B cells from P2, P6, P3, P5, H1, H4, H3, and H9, respectively. (B) (Lane M) A 100-bp ladder (Boehringer Mannheim). (Lane 1) A no-template control. (Lanes 2-5) A1 products (upper lanes) and β-actin products (lower lanes) generated using mRNA templates extracted from plasma cells from P3, P10, H12, and H13, respectively.