Compilation of Pax5 isoforms detected in untreated MM patients, treated MM patients, and healthy subjects.

P indicates patient; MM, multiple myeloma patient; H, healthy subject. Boxes represent detection of the Pax5 isoforms listed at left of diagram; hatched boxes for the Pax5 full-length isoform indicate that very low expression was detected in semiquantitative analyses. No attempts were made to semiquantitate any other isoforms, which were expressed at variable levels. (A) Isoforms detected by RT-PCR in B-cell populations from 5 healthy subjects, 6 untreated MM patients, and 7 treated MM patients. The isoforms shown for P11 and for P13 to P16 are also the isoforms that were observed in the CD22⁺ B-cell populations for these patients with one exception: The isoform missing exons 7-8 was not observed in the CD22⁺ cells of P14. (B) Isoforms detected by RT-PCR in plasma cells from 4 healthy subjects, 1 untreated MM patient, and 3 treated MM patients.