Induction of apoptosis by AN-9 in CEM cells.

CEM cells were treated with 60 or 75 μM AN-9 for 45 hours. Positive and negative control cells were treated with 5% ethanol and 0.1% DMSO, respectively. Cells were then stained with Alexa Fluor 488 annexin V and propidium iodide using the Vybrant Apoptosis Kit (Molecular Probes). Apoptotic cells were then determined by flow cytometry. Results are representative of 3 independent experiments.