Inhibition of colony formation by AN-9 of primary T-ALL and normal hematopoietic progenitors.

Primary T-ALL (n = 6) and normal bone marrow cells (n = 5) were cultured in methylcellulose medium and increasing concentrations of AN-9 for 14 days. The BFU-E was identified as a large aggregate of more than 64 hemoglobinized cells, or as clusters of 3 or more subcolonies consisting of 8 or more hemoglobinized cells per subcolony. The CFU-GM was enumerated as a group of more than 50 granulocytic/monocytic translucent cells. ▪ indicates T-ALL; ▴, CFU-GM; ○, BFU-E. Error bars represent standard error of the mean.