Inhibition of ³H-thymidine incorporation by AN-9 in primary ALL cells and normal bone marrow cells.

Normal bone marrow cells and primary ALL cells were treated with increasing concentrations of AN-9 for 3 days. Cells were then pulsed with ³H-thymidine for 6 hours. ● indicates T-ALL; ▪, B-precursor ALL; ♦, relapsed infant ALL; ⋄, diagnosed infant ALL; ▴, normal bone marrow. All experimental conditions were performed in triplicate. Error bars represent standard error of the mean for data obtained with normal bone marrow (n = 6) and primary T-ALL (n = 16). For data obtained with infant ALL (n = 1) and for a representative B-precursor ALL (n = 1), error bars represent standard deviation.