LIGHT rapidly translocates into caps of TCR and rafts on the surface of activated T cells.

BALB/c spleen T cells were cross-linked with anti-CD3 and anti-CD28 for 0 or 10 minutes. The locations of TCR (stained with biotin–anti-CD3 followed by Alexa 594–streptavidin in red), rafts (stained with Alexa-cholera toxin in red) and LIGHT (stained with TR6-Fc followed by Alexa 488–anti–human IgG in green) were revealed by confocal microscopy. Original magnification, ×600.