Acadesine-induced apoptosis is independent of PKA, ERK, JNK, and p38 MAPK.

Cells from B-CLL patients were untreated or treated with 0.5 mM acadesine in the absence or presence of 5 μM H-89 (H), 10 μM SP 600125 (SP), 10 μM U0126 (U), or 10 μM SB 220025 (SB), for 24 hours. All inhibitors were preincubated for 1 hour before the addition of acadesine. Viability was measured as described in “Patients, materials, and methods.” Data are shown as the mean value ± SD of 5 patients.