Expression of SOCS3 mRNA and protein in SOCS3 transformants of KT-1/A3.

(A) Total RNA was prepared from SOCS3 transformant clones, parental KT-1/A3, and mock clone and analyzed by Northern blotting for expression of SOCS3 mRNA. (B) Cell extracts were prepared and immunoblotted with anti-SOCS3 and antiactin antibody. Arrow indicates 27-kDa SOCS3 protein.