Expression of SOCS family genes in CML cell lines.

(A) KT-1/A3 and A3R cells were cultured and stimulated for 1 hour with or without 1000 U/mL IFN-α. Total RNA was prepared and subjected to RT-PCR analysis using specific primers. (B) Total RNA was prepared from cells treated with 1000 U IFN-α for 1 to 4 hours and analyzed by Northern blotting for expression of SOCS3 gene.