ALK-1QD inhibits HMVEC-d readhesion and spreading.

HMVEC-d's were infected (MOI = 10) with AdALK-1QD or Adβ-gal. At 15 hours after infection, cells were trypsinized and reseeded onto uncoated or coated (fibronectin, gelatin, collagen I, and collagen IV) plastic multiwell dishes. (A) Adherent cells were photographed 3 hours after reseeding under a phase contrast microscope (original magnification, × 100). (B) The number of adherent cells was determined with a particle counter 30 minutes after reseeding. (C) The number of fully spread versus partially spread and round cells were determined 30 minutes after reseeding on fibronectin.